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Receptors:
dopamine
Tumorigenic:
No
Age:
5 days
Comments:
The cells secrete prolactin and express functional dopamine receptors [U.S. Patent 5,510,260].
ATCC complete growth medium: The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium:
fetal bovine serum to a final concentration of 2.5%
horse serum to a final concentration of 15% Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C DGrowth Conditions: The line grows as clumps of cells in suspension.
Subculturing:
Protocol: Cultures can be maintained by addition or replacement of medium. Establish new cultures at 2 X 10(5) viable cells/ml and maintain between 1 X 10(5) and 1 X 10(6) cells/ml. Medium Renewal: Every 2 days
Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2004 recommended serum:ATCC 30-2020 recommended serum:ATCC 30-2040 Cell culture tested DMSO:ATCC 4-X
References:
22090: MacLeod RM, et al. Prolactin secreting cell line and method of obtaining the same. US Patent 5,510,260 dated Apr 23 1996
